Development and application of recombinant antibody-based immunoassays to tetraconazole residue analysis in fruit juices.
Identifieur interne : 000B38 ( Main/Exploration ); précédent : 000B37; suivant : 000B39Development and application of recombinant antibody-based immunoassays to tetraconazole residue analysis in fruit juices.
Auteurs : Emma Plana [Espagne] ; Maria-José Moreno ; Ángel Montoya ; Juan J. ManclúsSource :
- Food chemistry [ 0308-8146 ] ; 2014.
English descriptors
- KwdEn :
- Animals, Antibodies, Monoclonal (analysis), Antibodies, Monoclonal (genetics), Antibodies, Monoclonal (immunology), Beverages (analysis), Chlorobenzenes (analysis), Citrus sinensis (chemistry), Enzyme-Linked Immunosorbent Assay (methods), Food Contamination (analysis), Fruit (chemistry), Fungicides, Industrial (analysis), Malus (chemistry), Mice, Pesticide Residues (analysis), Triazoles (analysis).
- MESH :
- chemical , analysis : Antibodies, Monoclonal, Chlorobenzenes, Fungicides, Industrial, Pesticide Residues, Triazoles.
- chemical , genetics : Antibodies, Monoclonal.
- chemical , immunology : Antibodies, Monoclonal.
- analysis : Beverages, Food Contamination.
- chemistry : Citrus sinensis, Fruit, Malus.
- methods : Enzyme-Linked Immunosorbent Assay.
- Animals, Mice.
Abstract
Tetraconazole is currently used as a fungicide in fruit and vegetables. The aim of this work was the development of immunochemical techniques based on recombinant antibodies for the screening of tetraconazole residues in fruit juices. Recombinant antibodies were produced from a hybridoma cell line secreting a monoclonal antibody specific for tetraconazole and from lymphocytes of mice hyperimmunised with tetraconazole haptens conjugated to bovine serum albumin. From these antibodies, enzyme-linked immunosorbent assays in the conjugate-coated format were developed, which were able to detect tetraconazole standards down to 1ng/mL. From recovery studies with spiked samples, these immunoassays determined tetraconazole in orange and apple juices with acceptable reproducibility (coefficients of variation below 25%) and recoveries (ranging from 78% to 145%) for a screening technique. The analytical performance of RAb-based immunoassays was fairly similar to that of the MAb-based immunoassays. Due to their simplicity and high sample throughput, the developed recombinant-based immunoassays can be valuable analytical tools for the screening of tetraconazole residues in fruit juices at regulatory levels.
DOI: 10.1016/j.foodchem.2013.07.121
PubMed: 24054232
Affiliations:
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Le document en format XML
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Manclús</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:24054232</idno><idno type="pmid">24054232</idno><idno type="doi">10.1016/j.foodchem.2013.07.121</idno><idno type="wicri:Area/PubMed/Corpus">000384</idno><idno type="wicri:Area/PubMed/Curation">000384</idno><idno type="wicri:Area/PubMed/Checkpoint">000384</idno><idno type="wicri:Area/Ncbi/Merge">001435</idno><idno type="wicri:Area/Ncbi/Curation">001435</idno><idno type="wicri:Area/Ncbi/Checkpoint">001435</idno><idno type="wicri:doubleKey">0308-8146:2014:Plana E:development:and:application</idno><idno type="wicri:Area/Main/Merge">000B40</idno><idno type="wicri:Area/Main/Curation">000B38</idno><idno type="wicri:Area/Main/Exploration">000B38</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Development and application of recombinant antibody-based immunoassays to tetraconazole residue analysis in fruit juices.</title><author><name sortKey="Plana, Emma" sort="Plana, Emma" uniqKey="Plana E" first="Emma" last="Plana">Emma Plana</name><affiliation wicri:level="2"><nlm:affiliation>Instituto Interuniversitario de Investigación en Bioingeniería y Tecnología Orientada al Ser Humano, Universitat Politècnica de València, Camino de Vera s/n, Edificio 8B-N, 46022 Valencia, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto Interuniversitario de Investigación en Bioingeniería y Tecnología Orientada al Ser Humano, Universitat Politècnica de València, Camino de Vera s/n, Edificio 8B-N, 46022 Valencia</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté valencienne</region></placeName></affiliation></author><author><name sortKey="Moreno, Maria Jose" sort="Moreno, Maria Jose" uniqKey="Moreno M" first="Maria-José" last="Moreno">Maria-José Moreno</name></author><author><name sortKey="Montoya, Angel" sort="Montoya, Angel" uniqKey="Montoya A" first="Ángel" last="Montoya">Ángel Montoya</name></author><author><name sortKey="Manclus, Juan J" sort="Manclus, Juan J" uniqKey="Manclus J" first="Juan J" last="Manclús">Juan J. Manclús</name></author></analytic><series><title level="j">Food chemistry</title><idno type="ISSN">0308-8146</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Monoclonal (analysis)</term><term>Antibodies, Monoclonal (genetics)</term><term>Antibodies, Monoclonal (immunology)</term><term>Beverages (analysis)</term><term>Chlorobenzenes (analysis)</term><term>Citrus sinensis (chemistry)</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Food Contamination (analysis)</term><term>Fruit (chemistry)</term><term>Fungicides, Industrial (analysis)</term><term>Malus (chemistry)</term><term>Mice</term><term>Pesticide Residues (analysis)</term><term>Triazoles (analysis)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Chlorobenzenes</term><term>Fungicides, Industrial</term><term>Pesticide Residues</term><term>Triazoles</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antibodies, Monoclonal</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term></keywords><keywords scheme="MESH" qualifier="analysis" xml:lang="en"><term>Beverages</term><term>Food Contamination</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Citrus sinensis</term><term>Fruit</term><term>Malus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Mice</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Tetraconazole is currently used as a fungicide in fruit and vegetables. The aim of this work was the development of immunochemical techniques based on recombinant antibodies for the screening of tetraconazole residues in fruit juices. Recombinant antibodies were produced from a hybridoma cell line secreting a monoclonal antibody specific for tetraconazole and from lymphocytes of mice hyperimmunised with tetraconazole haptens conjugated to bovine serum albumin. From these antibodies, enzyme-linked immunosorbent assays in the conjugate-coated format were developed, which were able to detect tetraconazole standards down to 1ng/mL. From recovery studies with spiked samples, these immunoassays determined tetraconazole in orange and apple juices with acceptable reproducibility (coefficients of variation below 25%) and recoveries (ranging from 78% to 145%) for a screening technique. The analytical performance of RAb-based immunoassays was fairly similar to that of the MAb-based immunoassays. Due to their simplicity and high sample throughput, the developed recombinant-based immunoassays can be valuable analytical tools for the screening of tetraconazole residues in fruit juices at regulatory levels.</div></front></TEI><affiliations><list><country><li>Espagne</li></country><region><li>Communauté valencienne</li></region></list><tree><noCountry><name sortKey="Manclus, Juan J" sort="Manclus, Juan J" uniqKey="Manclus J" first="Juan J" last="Manclús">Juan J. Manclús</name><name sortKey="Montoya, Angel" sort="Montoya, Angel" uniqKey="Montoya A" first="Ángel" last="Montoya">Ángel Montoya</name><name sortKey="Moreno, Maria Jose" sort="Moreno, Maria Jose" uniqKey="Moreno M" first="Maria-José" last="Moreno">Maria-José Moreno</name></noCountry><country name="Espagne"><region name="Communauté valencienne"><name sortKey="Plana, Emma" sort="Plana, Emma" uniqKey="Plana E" first="Emma" last="Plana">Emma Plana</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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